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KMID : 0617819920020010231
Journal of Wonkwang Oriental Medicine
1992 Volume.2 No. 1 p.231 ~ p.248
Study on the Difference of the Saponin Content in the Gleditschia According to its Part and Collection Times


Abstract
On the whole, it has been known that the common effect by the part of the Gleditschia is the discharge of phlegm, the push of pus and the removal of tumor, the main component of which is the Gleditschia-saponin.
It has been reported that the component consisted in the Lehume and the Semin and did not exist in the Thorn.
This study was designed in the fact that the component will be contained in the Thorn which has been often used for the treatment of the push of pus and the removal of tumor, and in the fact that we could expect the good remedical value in the clinical treatment by comparing the content by its collection times, as the difference of the main component content by its collection times would differ in the remidical value.
Comparative study was made through analyzing Gleditshcia-saponin content by the MeOH extract and by the water extract according to various part and collection times of Gleditschia.
The result s were as follows:
1. As the result of the saponin confirmation reaction. both of the constant saopninfiable reaction and the Libermann-Burchard reaction proved positive.
2. The result confirmed of the Thin layer chromatograms showed that the sorts of the Gleditschia-saponin was eight and the Rf value of the Gleditschia-saponin by its part was the same.
3. As the result of measuring the Gleditschia-saponin content by MeOH extract, the Legume was highest by its part and the things gathered in December were highest by the collection times.
4. The result by the water extract showed that the Lehume was highest according to its part and the things gathered in December were highest by the collection times.
5. The content by the MeOH extract was higher than that by the water extract.
From the above results.
The Gleditschia-saponin was contained not only in the Legume and the Semen but also in the Thorn.
The content of Gleditschia-saponin was highest in December by the collection times.
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